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Key Facts Formulation (1X): 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄ Per litre: 8 g NaCl, 0.2 g KCl, 1.44 g Na₂HPO₄, 0.24 g KH₂PO₄ pH: 7.4, set before final volume adjustment Osmolality: about 280 to 300 mOsm/L Ca and Mg: absent from standard PBS and DPBS That is counterintuitive because PBS gets treated like background chemistry
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True as this may be, chronic FOXO4 reduction is not advisable as FOXOs play a role in DNA-damage repair and Foxo4−/− mice are susceptible to acute damage(Zhou et al., 2009)